Primers and probes

Primers and probes designed to be used in the molecular diagnosis of Dengue virus (four serotypes), Chikungunya virus, Zika virus, Orthobunyavirus (including Guaroa, Oropouche, Group C, Bunyamwera and California viruses), Influenza virus (Influenza A-matrix gene and B) and its subtypes (H3, H1pdm/SwH1N1, H1 seasonal), and SARS-CoV-2.

Primers and probes will be used in reverse transcription (RT) real-time PCR assays using serum samples from febrile surveillance protocol and nasopharyngeal swab samples from the respiratory surveillance protocol. The items have all been validated and are compatible with the equipment in use at NAMRU SOUTH: 7500 Fast Real-Time PCR and QuantStudio platforms.

INFA-FSC2-F1-100 Influenza A Multiplex Fwd Primer 1, 100 nmol - 2 quatities INFA-FSC2-F2-100 Influenza A Multiplex Fwd Primer 2, 100 nmol - 2 quantities INFA-FSC2-R1-100 Influenza A Multiplex Rev Primer 1, 100 nmol - 3 quantities INFA-FSC2-R2-100 Influenza A Multiplex Rev Primer 2, 100 nmol - 1 quantity INFA-FSC2-P-25 Influenza A Multiplex Probe, 25 nmol - 4 quantities INFB-FSC2-F-100 Influenza B Multiplex Fwd Primer, 100 nmol - 4 quantities INFB-FSC2-R-100 Influenza B Multiplex Rev Primer, 100 nmol - 4 quantities INFB-FSC2-P-25 Influenza B Multiplex Probe, 25 nmol - 4 quantities SC2-FSC2-F-100 SARS-CoV-2 Multiplex Fwd Primer, 100 nmol - 4 quantities SC2-FSC2-R-100 SARS-CoV-2 Multiplex Rev Primer, 100 nmol - 4 quantities SC2-FSC2-P-25 SARS-CoV-2 Multiplex Probe, 25 nmol - 4 quantities RNP-F-100 Human RNase P Extraction Control Forward Primer - 100 nmol - 4 quantities RNP-R-100 Human RNase P Extraction Control Reverse Primer - 100 nmol - 4 quantities RNP-FSC2-P-25 Human RNase P Multiplex Probe, 25 nmol - 4 quantities DLO-CAB2-2 Dual-labeled Probe, 5' CAL Fluor Red 610/3' BHQ -2 200 nmol - 7 quantities DLO-COB1-2 Dual-labeled Probe, 5' CAL Fluor® Orange 560/3' BHQ-1 200 nmol - 5 quantities DLO-FB1-2 Dual-labeled Probe, 5' FAM/3' BHQ-1 200 nmol - 33 quantities DLO-FB1-2sp Dual-labeled Probe, 5' FAM/1 Internal Inosine/3' BHQ-1 200 nmol - 10 quantities DLO-FBP-2 5’ FAM BHQplus Probe Provides 20 nmol delivered - 10 quantities DLO-Q6B2-2 Dual-labeled Probe, 5' Quasar 670/3’ BHQ-2 200 nmol - 5 quantities LNA-FB1-20 Dual-labeled LNA Probe, 5' FAM/3' BHQ-1 200 nmol - 10 quantities PPC-2 Primer, (with probe purchase) Cartridge Purified (up to 30 bases each) 200 nmol - 118 quantities DNA-2 Custom Oligonucleotide, 21 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol - 5 quantities DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol - 5 quantities DNA-2 Custom Oligonucleotide, 22 Bases, 1 Internal Inosine, Cartridge Purified 200 nmol - 5 quantities DNA-2 Custom Oligonucleotide, 18 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities DNA-2 Custom Oligonucleotide, 20 Bases, 2 Internal Inosines, Cartridge Purified 200 nmol - 5 quantities PP-2 Primer, (with probe purchase) Salt-Free (up to 30 bases each) 200 nmol - 62 quantities P1-2 Primer, Avg. 26 Bases, Salt-Free 200 nmol - 246 quantities