Genome Wide Functional Genomics CRISPR KO Screening Techniques

The purpose of this procurement is to use Cellecta’ s Next Generation Sequencing (NGS) Services to sequence DNA samples produced as the result of CRISPR screens, for the Functional Genomics Lab. We require Cellecta to conduct NGS on the submitted samples and provide us with gene counts to determine which genes regulate the survival of wild type versus topoisomerase depleted colorectal cancer cells to elucidate mechanisms of resistance.

NCATS will provide Vendor with 15 extracted gDNA samples from our genome-wide CRISPR KO Screen and Cellecta will then amplify and sequence that DNA by NGS with an average depth of ~500 reads/guide sgRNA distributions from genomic DNA isolated from each sample and the deliverable will consist of a spreadsheet report with sgRNA read counts. Sequencing technicians will require 6-8 weeks to amplify DNA, sequence it and for their bioinformatician to report the sequencing counts