# Genome Wide Functional Genomics CRISPR KO Screening Techniques

Canonical: https://abierto.us/opportunities/25003450

- Solicitation number: 25-003450
- Notice type: Combined synopsis and solicitation
- Status: Closed. Deadline was July 24, 2025 at 9:00 AM EDT
- Department: Department of Health and Human Services
- Agency: National Institutes of Health
- Contracting office: National Institutes of Health Ncats (75N95C)
- NAICS: 541380 Testing Laboratories and Services
- Product or service code: Q301 Reference Laboratory Testing
- Place of performance: Mountain View, California
- County: Santa Clara County (FIPS 06085). https://abierto.us/counties/santa-clara-county-ca-06085
- First posted: July 17, 2025
- Last posted: July 17, 2025
- SAM.gov: https://sam.gov/workspace/contract/opp/23167e2daa454a86941ccda86451318a/view

## Description

The purpose of this procurement is to use Cellecta’ s Next Generation Sequencing (NGS) Services to sequence DNA samples produced as the result of CRISPR screens, for the Functional Genomics Lab. We require Cellecta to conduct NGS on the submitted samples and provide us with gene counts to determine which genes regulate the survival of wild type versus topoisomerase depleted colorectal cancer cells to elucidate mechanisms of resistance.

NCATS will provide Vendor with 15 extracted gDNA samples from our genome-wide CRISPR KO Screen and Cellecta will then amplify and sequence that DNA by NGS with an average depth of ~500 reads/guide sgRNA distributions from genomic DNA isolated from each sample and the deliverable will consist of a spreadsheet report with sgRNA read counts. Sequencing technicians will require 6-8 weeks to amplify DNA, sequence it and for their bioinformatician to report the sequencing counts

## Publications

- July 17, 2025: Combined synopsis and solicitation, due July 24, 2025 at 9:00 AM EDT. Notice 23167e2daa454a86941ccda86451318a. https://sam.gov/workspace/contract/opp/23167e2daa454a86941ccda86451318a/view

## Points of contact

- Brian Lind, brian.lind@nih.gov, 3015498939

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Source: SAM.gov Contract Opportunities bulk extract. Confirm deadlines on SAM.gov before responding. Cite https://abierto.us/opportunities/25003450.