QUANTSTUDIO 5 384 QPCR SYSTEM

AMENDMENT ONE

The purpose of this Amendment is to answer vendor questions. No further questions will be accepted. QUOTES Quotes must be submitted FOB Destination shipping to Boise, ID, 83702. Quotes are due Tuesday, August 4, 2026, at 10:00am PD. Quotes evaluated by Lowest Price Technically Acceptable (LPTA) Please include lead time in your quote. Quotes accepted by email to the Contracting Officer, Elizabeth Adam, at elizabeth_adam@ios.doi.gov only.

QUESTIONS and ANSWERS 1. The salient characteristics require an average sample ramp rate of 3.66°C/sec or greater. Thermo Fisher's published specification for the QuantStudio 5 384-well system states an average sample ramp rate of 2.92°C/sec. Since this is a brand-name-only procurement, please confirm whether 2.92°C/sec is acceptable or whether the requirement will be corrected? This is acceptable. 2. The brand-name justification refers to independently programmed temperature zones across the heat block.

Thermo Fisher's published literature indicates that VeriFlex temperature zones are not applicable to the 384-well QuantStudio 5 configuration. Please confirm whether independently programmable temperature zones are a mandatory requirement? This is not required. 3. Please confirm the required Thermo Fisher catalog number and whether the requested configuration must include a laptop or desktop computer? This should not include a computer.

Catalog number: A28140 4. Are installation, calibration, onsite setup or user training required? No 5. Is the manufacturer's standard warranty acceptable, and is any extended service coverage required? Standard warranty is acceptable 6. Must the successful contractor be an authorized Thermo Fisher reseller, and should evidence of authorization be included with the quotation?

Yes 7. Please confirm whether a manufacturer-issued VPAT/Accessibility Conformance Report and supporting accessibility narrative will satisfy the Section 508 submission requirements. Yes

Offerors are to include a technical description of the items being offered in sufficient detail to evaluate compliance with the requirements in the solicitation as seen in the Salient Characteristics (see Attachment). This may include product literature or other documents, if necessary, and is to include the country of manufacture. Offerors shall return the following:

Signed SF-18 Signed Amendment Firm, fixed price quote Product information System for Award Management (SAM) Unique Identification Entity (UEI)