Instrumental Sample Analyses

Objectives: The contractor shall perform molecular signaling analyses using peptide/protein measurement via HPLC, LC-MS analyses which will allow researchers to determine the best method to produce optimal signaling within a colony and will support model development; phage particle measurement using Transmission Electron Microscopy/Scanning Electron Microscopy (TEM/SEM) analyses which will be used to describe and characterize phage isolates; cyanobacteria inhibition using peptide/protein measurement using via HPLC, LC-MS analyses to determine effectiveness of plasmids containing genes to alter circadian rhythm of cyanobacteria; and trehalose measurement via High Performance Liquid Chromatography (HPLC) and/or Liquid Chromatography-Mass Spectrometry (LC-MS) analyses to determine the impact on cell survival during desiccation.

Deliverables include monthly progress reports, methods used, and results (data). The data obtained from these analyses will allow researchers to determine the best method to produce optimal signaling within a colony and determine optimal parameters to support model development.