Replacement of Non-functional Nikon TIRF Microscope – LCMB Microscopy Core Facility, NIH/NCI

PRODUCT FEATURES/SALIENT CHARACTERISTICS The following product features and characteristics are required for this requirement: Ti2E Motorized PFS Microscope Bundle with motorized encoded stage and fluorescence filter sets (DAPI, GFP, DS Red) Nikon Motorized E-TIRF Package with Ti2-P-FWB-E barrier filter wheel ORCA-FusionBT sCMOS camera with USB3.0 interface card and dual camera interface kit NI-BB Plus analog triggered device hub LUNF STORM 405/488/561/640 multi-line laser unit Ti2 motorized main branch and sub branch with exchangeable 100% reflective mirror C-NSTORM Quad 405/488/561/640 filter set Ti2-NS2-MU N-STORM magnification change unit NIS-Elements software package with Advanced Research licenses (×6) and HASP reconfiguration Z4 Advanced Imaging Workstation V8 with Thunderbolt 31.5-inch 4K display Installation kit Reintegration of the following existing components from the current instrument at no additional cost to CCR/NCI: CFI60 Apochromat TIRF 100X Oil Immersion Objective Lens (N.A.

1.49), MCL 100 micrometer piezo nanopositioner with controller and electronics, EMCCD camera, C-NSTORM 647 filter set, C-FL TIRF CFP/YFP filter set, and NIS-Elements STORM acquisition and analysis software licenses DELIVERY / INSTALLATION Contractor shall deliver all system components within 90–120 days after award to:

Sandeep Pallikkuth, LCMB, CCR, NIH/NCI 37 Convent Dr. MSC 4256 Building 37, Room 2033B1 Bethesda, Maryland 20892 Upon award and delivery, the contractor must notify the NCI Project Officer, Sandeep Pallikkuth, to schedule an installation date and time. Installation must include full optical alignment, integration of all new components, reintegration of reused components from the existing instrument, and verification of complete system performance to manufacturer specifications.

The Contractor shall install the system and have it ready for use within fourteen (14) days after delivery. 4.3 TRAINING Onsite training of LCMB Microscopy Core personnel on operation of the complete system is required following installation.