dCas9-KRAB Cell Lines

The solicitation number is 75N95024Q00213 and the solicitation is issued as a request for quotation (RFQ). This acquisition is for a commercial item or service and is conducted under the authority of the Federal Acquisition Regulation (FAR) Part 13—Simplified Acquisition Procedures; and FAR Part 12—Acquisition of Commercial Items, and is not expected to exceed the simplified acquisition threshold.

The associated NAICS code is 541380 – Testing Laboratories and Services and the small business size standard is $19.0 million. This requirement is full and open with no set-aside restrictions. This requirement is for the following services: The National Institutes of Health has established an RNAi/CRIPSR Cas9 screening facility to provide intramural researchers with access to the equipment and expertise necessary to conduct genome-wide RNAi/ CRIPSR Cas9 screens in mammalian cells.

The NCATS Functional Genomics Lab requires six (6) dCas9 cell lines that will be used to repress the target gene expression when applied in a CRISPRi screen. See attached Statement of Work.