Sequencing instrument_DNA and RNA sample analysis

THE PURPOSE OF THIS AMENDMENT IS TO PROVIDE QUESTIONS WITH ANSWERS. The U.S. Geological Survey, Eastern Ecological Science Center, located in Laurel MD, is seeking to procure a sequencing instrument for DNA and RNA sample analysis. The instrument specifications are as follows:

sequence DNA or RNA fragments from 20 bases to >4 million bases; direct, PCR-free sequencing of native DNA and/or RNA; built-in methylation detection; direct electronic analysis of the target molecule, rather than sequencing a synthetic copy or using surrogate markers such as fluorescence; duplex data > Q30 (>99/9%) and perfect reads from DNA molecules 10s of kilobases in length; and, real-time sequencing.

Quotes shall provide pricing and itemized component descriptions that meet or exceed the Specifications stated above. This will be a firm fixed-price contract. Quote submissions are due on or before 12/22/2023. The anticipated award date is 1/11/2024. The acquisition is a total small business set-aside. The NAICS code is 334516 and size standard is 1,000 employees. Vendors must be registered at https://www.sam.gov/ to be awarded the contract. All responsible sources may submit a quotation which shall be considered by the government. All correspondence and quotes MUST be emailed to sruggles@usgs.gov.