# scRNA sequencing service

Canonical: https://abierto.us/opportunities/nibib101

- Solicitation number: NIBIB101
- Notice type: Sources sought
- Status: Closed. Deadline was August 4, 2025 at 8:00 AM EDT
- Department: Department of Health and Human Services
- Agency: National Institutes of Health
- Contracting office: National Institutes of Health Ninr (75N98F)
- Place of performance: Bethesda, Maryland
- County: Montgomery County (FIPS 24031). https://abierto.us/counties/montgomery-county-md-24031
- City: Bethesda. https://abierto.us/cities/bethesda-md-2407125
- First posted: July 29, 2025
- Last posted: July 29, 2025
- SAM.gov: https://sam.gov/workspace/contract/opp/c93c173291de4ab9bbb17c9930d5ebdf/view

## Description

The system utilizes a microfluidics based methods of sequencing. . The workflow includes dead cell removal to ensure high data quality. . The sample preparation workflow includes a protocol for maximizing cell viability. . The sequencing system has .80% of bases with quality scores of Q30 or higher for 2x150 bp sequencing with an average that is preferably .90%. . Workflow includes barcoding cells and droplet isolation using droplet sequencing methods. .

System utilizes semiautomated processing for surface marker enrichment and cell isolation. . Sequencing workflow includes end repair, A-tailing, ligation and PCR. . System provides flexible sequencing depth with a capture rate > 60%. . System capable of profiling paired V(D)J transcripts with 5?fgene expression and library preparation. . Contractor provides a robust bioinformatics pipeline for visualization and cross referencing of sequencing data.

## Publications

- July 29, 2025: Sources sought, due August 4, 2025 at 8:00 AM EDT. Notice c93c173291de4ab9bbb17c9930d5ebdf. https://sam.gov/workspace/contract/opp/c93c173291de4ab9bbb17c9930d5ebdf/view

## Points of contact

- Roberta Albert, roberta.albert@nih.gov

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Source: SAM.gov Contract Opportunities bulk extract. Confirm deadlines on SAM.gov before responding. Cite https://abierto.us/opportunities/nibib101.